Analysis and interpretation of enzyme kinetic data

[email protected] Abstract Analysis of enzyme kinetic data to obtain valid information requires attention to two details that are often given less attention than they need. The first is an experimental design that ensures that the variables treated as independent are truly independent, that different interpretations can be distinguished, and that parameter values can be estimated. The second is that authors should be aware of the statistical assumptions that are implicit in the fitting programs that they use, whether commercial or not. & 2014 The Author. Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).